Laser Micro Dissection Systems: An Alternative Method of Defined Cell Isolation for Molecular Profiling
نویسنده
چکیده
Laser micro-dissection permits isolation groups of cells from heterogeneous tissue sections under direct microscopic visualization. This technology not only allows isolation of homogenous subpopulation of cells but also allows immunophenotypically similar cells for RNA/ DNA or protein extraction for downstream applications like western blot, mass spectrophotometry, real time quantitative polymerase chain reaction, microarray and RNA sequencing. Isolation of dead cells unsuitable for functional assays, high cost of equipment and technical expertise required for operation are few limitations to laser microdissection systems.
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